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KMID : 0385520090220050407
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Analytical Science & Technology
2009 Volume.22 No. 5 p.407 ~ p.414
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Simultaneous quantitative determination of urinary cholesterol, desmosterol and lanosterol in pravastatin treated rats by gas chromatography/mass spectrometry
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Bhowmik Salil Kumar
Chung Bong-Chul
Lee Young-Joo
Yi Hong-Jae
Jung Byung-Hwa
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Abstract
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A simultaneous determination method for cholesterol, lanosterol and desmosterol was developed using gas chromatography/mass spectrometry. Urine was enzymatically hydrolyzed with β-glucuronidase/arylsulfatase. Samples were prepared using extractions with a mixture of ethyl acetate-hexane (2:3, v/v), followed by derivatization with a mixture of MSTFA/TMSI/TMCS (100:2:5 v/v/v). All analyses were performed using GC/MS in selective ion monitoring mode. Good linearities (r²=0.998~0.999) in calibration curve and a satisfactory recovery (80.0%~113%) were achieved. Accuracy and precision values within ±15% in the concentration range of 5 to 200 ng/mL were also observed for all compounds. The developed method was applied to pravastatintreated (70 and 250 ㎎/㎏/day for 7 days, oral) hyperlipidemia rats. Those sterols were significantly lower in drug-treated rats compared to the controls, which justifies the drug efficacy. Therefore, these results indicate that the developed method was successfully applied to examine statin drug efficacy with urine sample.
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KEYWORD
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cholesterol, desmosterol, lanosterol, pravastatin, GC/MS
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